An overview of three-dimensional cell culture

What is 3D cell culture?

Three-dimensional cell culture is based on the common three-dimensional culture model of the scaffold, which can better simulate the natural environment in which the cells grow. Three-dimensional cell culture (TDCC) refers to the co-culture of vectors with different materials in three dimensions and various kinds of cells in vitro, so that cells can migrate and grow in the three-dimensional spatial structure of the carrier. Three-dimensional cell-carrier complex.

Frontier knowledge about 3D cell culture research

  1. Three-dimensional cell tissue plus tensile culture model of the United States flexcell company Tissue Train tensile stress stimulation three-dimensional hydrogel stent cell tissue culture system.

Functional highlights of the three-dimensional cell tissue augmentation culture model: after burning three-dimensional cell tissue culture and stretching three-dimensional cell tissue culture in the true sense of three-dimensional culture – the system with a variety of coated surfaces (Amino, Collagen (Type I Or IV), Elastin, ProNectin (RGD), Laminin (YIGSR) collagen hydrogels for extracellular matrix scaffolds in biomaterial scaffold studies, compared to traditional nanofiber scaffolds and porous scaffolds, hydrogel scaffolds The network contains a lot of water, which can supply cell nutrients well, and can also cross-link bioactive factors to regulate cell growth and differentiation. Therefore, hydrogel scaffolds can better simulate the tissue-like physics required for cell growth. The spatial structure has high plasticity, relatively simple manufacturing process and convenient clinical application.

  1. Three-dimensional cell tissue pressure-carrying culture system model of American three-dimensional cell tissue after-force culture model

Three-dimensional cell culture in three-dimensional cell culture

1) The system provides periodic or static pressure loading of various tissue, three-dimensional cell cultures;

2) based on the deformation of the flexible film substrate, uniform force;

3) Real-time observation of the reaction of cells and tissues under pressure;

4) can selectively block stress loading on cells;

5) Simultaneously have multi-channel cell pull force loading function;

6) Up to 4 channels, 4 different programs can be run simultaneously, and multiple different pressure deformation rate comparison experiments are performed;

7) Multiple frequencies (0.01-5 Hz), multiple amplitudes and multiple waveforms can be operated in the same program;

8) Better control of waveforms under ultra-low or ultra-high stress;

9) A variety of waveform types: static waveform, positive rotation waveform, cardiac waveform, triangular waveform, rectangle and various special waveforms;

10) computer system for pressure loading cycle, size, frequency, duration precise intelligent control typical application range: detection Biochemical reactions of various tissues and cells under pressure.

  1. Three-dimensional cell tissue augmentation culture model. Three-dimensional cell tissue stretchestensile force loading culture system model of American flexcell company Three-dimensional cell culture three-dimensional cell tension loading culture

1) The system provides axial and circumferential stress loading on two-dimensional, three-dimensional cells and tissues;

2) based on the deformation of the flexible film substrate, uniform force;

3) The reaction of cells and tissues under stress can be observed in real time;

4) can selectively block stress loading on cells;

5) Simultaneous multi-channel cell pressure loading function;

6) In combination with the Flex Flow parallel slab flow chamber, fluid shear stress can be applied while pulling the cells;

7) Up to 4 channels, 4 different programs can be run simultaneously, and multiple different tensile deformation rate comparison experiments are performed;

8) Multiple frequencies, multiple amplitudes and multiple waveforms can be operated in the same program;

9) Better control of waveforms under ultra-low or ultra-high stress;

10) Multiple waveform types: static waveform, positive rotation waveform, cardiac waveform, triangular waveform, rectangle, and various special waveforms;

3 Three-dimensional cell cultures in life applications:

Customized 3D cell culture services are common in our lives, and there are also 3D cell culture related products on the market.

  • Creative Bioarrayoffers 35 human cell systems with over 160 different cell types. Moreover, we also provide our customers primary cells from over 13 types of other animals.
  • These cells taken from living tissue are extremely accurate as they are literally coming from the source and are available from many sources on the human body. These living samples can give extremely accurate information about the cells in vivo and give relevant information regarding the living systems.
  • Not only canCreative Bioarray offer such a wide range of primary cells of humans, but we also have a selection of primary cells of animals for comparative testing.

Bone cells, which are found within the bone tissue, are responsible for bone production, maintenance and modeling. There are three different types of cells that found only in the bone. The osteoblasts are derived from mesenchymal stem cells and its function is bone matrix synthesis and its subsequent mineralization. The osteoclasts are large cells that dissolve the bone and osteocytes are cells inside the bone. At Creative Bioarray, we offer 6 types of human primary bone cells including: Human Bone marrow-derived endothelial Cells, Human Osteoblast Cells, Human Osteoblasts (HOB), Human Osteoblast Cells (Postnatal), Human Calvarial Osteoblasts (HCO) and Human Osteoblasts-femural (HO-f). The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody. Human osteoblasts may be used for various types of in vitro, in vivo, or regenerative medicine studies in normal or diseased systems. In addition, they may be used in bone development studies.